Phenolic acid content and antioxidative capacity of methanolic extracts from quality Indian mustard genotypes

H Punetha and Ankur Adhikari


Brassica is one of the foremost oilseed crop cultivated in several parts of the world. Its oil is consumable and defatted
meal is a good element for use in aquaculture, animal and human diets, which acquires a copious amount of healthenhancing
phytochemicals and anticarcinogenic compounds such as glucosinolates and antioxidative principles such
as phenolics, flavonoids, vitamin- C & E. The present investigation emphasized on the phenolic content and antioxidative
activity in 30 germplasm of Brassica juncea. It is catechizing that methanolic extracts of a defatted meal of PRQ-2005-
11(BE) (24.14 mg/ml) exhibit paramount in-vitro antioxidative property and F3-F4(3) (6.48 mg/g) possessed utmost total
phenolic content. Ortho dihidroxyphenol content differ from 0.070 mg/ml (LG 2005-36-1) to 0.323 mg/ml (F3-F4(2)). The
reducing power activity was observed to be highest in PLGM-2002-2-7(1.722) followed by PRQ-2005-11-BE (1.674 mg/
ml), DCF4-F5-3 (1.672 mg/ml) in a dose-dependent manner. The predominant free radical-scavenging activity was observed
in DCF4-F5(3) (84.27 mg/ml) at the concentration of 800 μg/ml. Whereas the heightened chelating activity was found in
PRQ-2005-4(BL) (71.81 mg/ml) followed by PRQ-2004-9 (71.54 mg/ml) and PDLM 2003-9-6 (70.23 mg/ml) at the concentration
of 800 μg/ml. Thus the defatted meal of 30 Indian mustard genotypes was found to possess rich antioxidative capacity
which further utilized in the preparation of functional foods as a viable food ingredient.


Antioxidative, Indian mustard, phenolics, quality

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