Molecular and morphological diversity of the mori CMS lines and restores in Indian mustard (Brassica juncea L.)

Raju Ram Choudhary*, Ram Avtar, Pawan Kumar, Manjeet Singh and Mahavir Bishnoi


Indian mustard represents one of the most important oilseed crops in India, nevertheless, their genetic diversity is barely known. A better understanding on this topic is essential for the proper utilization of genotypes in different target breeding programmes. We evaluated the genetic diversity among 15 Indian mustard (Brassica juncea L.) genotypes including 10 mori CMS lines and five restorers of mori CMS. For this, we used A and B genome specific SSR markers and phenotypic data on yield and yield contributing traits. Out of the 35 primers tested, 15 reported polymorphic and a total of 38 alleles were amplified. The range of PIC value was 0.11-0.71 with mean value of 0.36 demonstrating moderate discriminating capability of SSR markers used for this study. Cluster analysis for SSR profile was performed using DARwin v 6.0 and for morphological traits was performed using WARD’s method through Euclidean distance estimation by JMP v.14 software. Genotypes were grouped into four clusters based on genetic distances and into three clusters based on morphological dissimilarity. Three SSRs in our study have PIC values of more than 0.50 suggesting their effectiveness in distinguishing studied genotypes. The use of genotypes from different cluster in hybridization is assuring to produce feasible heterotic combinations, which will be helpful in development of superior cross combination.


Genetic diversity, mori CMS, phenotypic diversity, SSR markers, principal coordinates

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